Table 1: Tetrazolium (TTC) rating and in vitro germination response of cut seeds and embryos of eighteen aged Cucurbita pepo accessions in three squash rescue (SR) medium treatments. Treatment 1 consisted of SR medium in gel form poured on petri dish (SR-G), treatment 2 consisted of SR medium in gel form poured on sterile towels (SR-GT), while treatment 3 consisted of liquid SR medium poured on sterile towel (SR-LT). For SR-G treatment, both cut seeds and embryos were plated, while as for SR-GT and SR-LT, only cut seeds were plated.

Accession Seed count Empty seeds (%) TTC score Contamination (%) Germination (%)
SR-G SR-GT Cut-seed SR-LT Cut-seed Average per accession
Cut-seed Embryo
C374 67 0.04 1 25 11.11 20.00 0 5.56 9.17
NY247 8 0 - 100 - 0 - - 0
NY247op 39 0.08 0 66.67 0 0 0 0 0
PKC2116 76 0.01 1 0 40.00 100 90.00 80.00 77.50
S286 51 0.04 0 4.08 78.57 28.57 78.57 50.00 58.93
S347 62 0.05 3 1.69 94.12 87.50 72.22 93.75 86.90
S387 81 0 1 19.75 12.5 0 16.67 33.33 15.63
S403 90 0.01 3 4.49 80.77 90.91 7.69 42.31 55.42
S421 110 0.04 2 0 100 40.00 88.24 91.18 79.85
33B 52 0 3 0 83.33 93.75 100 91.67 92.19
S971 74 12.16 1 49.23 0 0 0 0 0
YSN180 68 16.18 0 12.28 0 0 0 0 0
YSN818 61 0.03 1 13.56 12.50 10.00 0 5.88 7.10
Z33 44 0.02 1 18.60 0 0 0 0 0
Z77 44 0.02 1 20.93 0 0 0 0 0
Z106 48 0.06 2 11.90 26.67 12.50 11.11 10.00 15.07
Z153 88 0.01 3 2.30 76.92 100 19.23 61.54 64.42
Z198 35 0.11 1 32.26 0 20.00 0 0 5